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The destabilization of the endosomal membrane is thought to be facilitated by the helper lipid [Farhood et al, 1992], enabling the DNA to escape degradation [Zhou X and Huang, 1994; Crystal, 1995] The DNA is thereafter released into the cytoplasm, where it enters the nucleus through the nuclear pores by an unidenti ed mechanism [Seisenberger et al, 2001] Cationic liposomes that are currently used to transfect a variety of cell lines and primary cells are the monocationic cholesterol derivative DC-Chol [Gao and Huang, 1991], the polycationic lipid DMRIE [San et al, 1993], DOTMA (part of Lipofectin ) [Felgner et al, 1987], and DOSPA (part of Lipofectamine ) [Felgner et al, 1995] Transfection of chondrocytes of avian origin has been successful with cationic liposomes like Lipofectin [Long and Linsenmayer, 1995] or Lipofectamine [Pallante et al, 1996].

luminance component. To exploit these characteristics the chrominance components are low-pass ltered and subsampled by a factor of 2 along with the horizontal and vertical dimensions, thus producing chrominance components that are one-fourth the spatial resolution of the luminance components.

However, transfection by these methods with bovine and human articular chondrocytes has not been forthcoming Recently, nonliposomal lipid-mediated methods have been described to offer high transfection ef ciencies in primary chondrocytes Among them, FuGENE 6, a nonliposomal mixture of lipids and other components, has been particularly effective [Madry et al, 2001, 2002, 2003a; Madry and Trippel, 2000; Lefebvre et al, 1998; Dinser et al, 2001] The protocol outlined below will focus on transfection with FuGENE 6..

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Indeed, a person is always in some state of core affect, which can be extreme, mild, or even neutral Core affect is part of most psychological processes Speci cally, core affect is one part of those events people call emotion (and which we call emotional episodes) Selfreports of emotion persistently yield two large general factors interpretable as pleasure and arousal (eg, Russell & Mehrabian, 1977; Watson & Clark, 1992) Furthermore, the manipulation of arousal by drugs in uences self-reported discrete emotions (Cooper, Zanna, & Taves, 1978; Gerdes, 1979; Schachter & Latan , 1964; Schachter & Wheeler, 1962) Feldman Barrett and Russell (2000) explored this hypothesis further in a study of self-reported emotions In one condition participants were asked to describe how they currently felt In a second condition they were asked to search their memory for the very last time they had an emotion.

Blackwell handbook of social psychology: Interpersonal processes (pp. 359 387). London: Blackwell. Rusbult, C. E., & Martz, J. M. (1995). Remaining in an abusive relationship: An investment model analysis of nonvoluntary commitment. Personality and Social Psychology Bulletin, 21, 558 571. Rusbult, C. E., & Van Lange, P. A. M. (1996). Interdependence processes. In E. T. Higgins & A. Kruglanski (Eds.), Social psychology: Handbook of basic principles. New York: Guilford Press. Rusbult, C. E., Verette, J., Whitney, G., Slovik, L., & Lipkus, I. (1991). Accomodation processes in close relationships: Theory and preliminary empirical evidence. Journal of Personality and Social Psychology, 60, 53 78. Sabatelli, R. M., & Cecil-Pigo, E. F. (1985). Relational interdependence and commitment in marriage. Journal of Marriage and the Family, 47, 931 937. Simpson, J. A., Gangestad, S. W., & Lerma, M. (1990). Perception of physical attractiveness: Mechanisms involved in the maintenance of romantic relationships. Journal of Personality and Social Psychology, 59, 1192 1201. Simpson, J. A., & Rholes, W. S. (1998). Attachment theory and close relationships. New York: Guilford Press. Simpson, J. A., Rholes, W. S., & Nelligan, J. S. (1992). Support seeking and support giving within couples in an anxietyprovoking situation: The role of attachment styles. Journal of Personality and Social Psychology, 62, 434 446.

where only the term e 2 k=1 (yik xik ) is signi cant because all the other terms in this expression are constants. The expression for calculating coef cients i (u , u) is nally i (u , u) = C ebi L(bi )/2 e 2

In the third condition they were asked to search their memories for a strong, clear emotion In all three conditions the pleasure and arousal dimensions accounted for substantial variance in the intensity of self-reported emotions However, as the event to be described became more restricted to clearer and stronger cases of emotion, the amount of variance.

Shrout, P, 455, 459, 542 Shulhan, D, 413 Shumaker, S, 142, 174, 189 Shuster, J, 345, 359 Shuttleworth, E C, 92 Shutty, M, 432 Sichel, D S, 538 Sicks, J D, 495, 509 Sicotte, C, 515, 538 Siddall, P J, 421, 435 Siddiqui, F, 433 Sidney, S, 353, 354, 361, 508 Sidot, S, 5, 12, 13, 22 Sieber, M, 68 Sieber, S D, 530, 543 Siegel, B S, 33, 282, 288 Siegel, C I, 398, 411 Siegel, J M, 33, 48, 396, 412, 470, 485 Siegel, K, 66, 73 Siegel, S, 451, 461 Siegfried, W, 112, 118 Siegler, I C, 346, 357, 363, 364, 487, 488, 489, 491, 496, 497, 498, 499, 500, 503, 504, 508, 509, 536, 543, 545, 560 Siegman, A W.

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